Bioreactor

Description

Fumaric acid and ethanol are produced through the aerobic fermentation of glucose by Rhizopus oryzae. Ethanol is an unwanted by-product and its concentration is reduced by sparging with carbon dioxide. There are two phases in the process: a growth phase and production phase. During the growth phase, the fungus is grown on a PVC pipe within the cylindrical fermentation vessel with high concentrations of urea in a batch process. Thereafter, in the production phase, the vessel is rinsed out and continuous fermentation begins with lower concentrations of urea.

Mixing in the vessel is caused by the continuous recycling of the reactor fluid from the base to the head of the reactor and as such the system is modelled as a CSTR. The direction of the recycle switches periodically to prevent the build-up of fungus and subsequent clogging of the pump. Volume is kept constant through the use of an overflow. The overflow's fumaric acid, glucose and ethanol concentrations is measured.

Previous work

Andre Naude (2018)

On the bio side much work has been done by Naude who did his PhD on the reactor in 2018. The three published papers relating to his work can be found here, here, and here. His full PhD thesis can be found here

Reuben Swart (2019)

Swart also worked on the bio side and has completed his Masters on the reactor. His work can be found [here]

Darren Roos (2019)

Roos worked on the control side. He characterized the pH probe that is used in the reactor in terms of its accuracy, linearity, drift and dynamic response. The probe (if recently calibrated) is both accurate to within 0.045% and linear throughout the range of 2 pH to 11 pH. 24h drift experiments found that the average drift of the measurement is 0.01375 pH per hour. Dynamic tests found a first order system with a gain of one and time constant of 130 seconds described the response well.

He also developed a nonlinear model of the system that is used in state estimation. An Unscented Kalman Filter is used in the state estimation system. A useful article for understanding how it works can be found here.